| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : |
Rabbit monoclonal anti-Wee1-Like Protein Kinase for use in WB, IHC, IF, IP and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : |
Monoclonal |
| Clone ID : |
PT1356R |
| Conjugation: |
Unconjugated |
| Isotype: |
IgGk |
| Formulation: |
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification: |
Protein A |
| Dilution Range: |
IHC 1:100-1:200WB 1:2000-1:10000IF 1:200-1:1000ELISA 1:5000-1:20000IP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Protein Name | Wee1-Like Protein KinaseWee1huWee1a Kinase |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69202Reactome: R-HSA-69273Reactome: R-HSA-69478Reactome: R-HSA-69656Reactome: R-HSA-75035Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Wee1-Like Protein Kinase antibodyAnti-Wee1hu antibodyAnti-Wee1a Kinase antibodyAnti-WEE1 antibody |
Information sourced from Uniprot.org
