Anti-WDR45 antibody (35-148) (STJ29718)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal WDR45 (35-148) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WDR45 |
| Gene ID | 11152 |
| Uniprot ID | WIPI4_HUMAN |
| Immunogen Region | 35-148 |
| Immunogen Sequence | EPLMEKGHLDHEQVGSMGLV EMLHRSNLLALVGGGSSPKF SEISVLIWDDAREGKDSKEK LVLEFTFTKPVLSVRMRHDK IVIVLKNRIYVYSFPDNPRK LFEFDTRDNPKGLC |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 35-148 of human WDR45 (NP_001025067.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed, with high expression in skeletal muscle and heart. Weakly expressed in liver and placenta. Expression is down-regulated in pancreatic and in kidney tumors. |
| Function | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Together with WIPI1, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Probably recruited to membranes through its PtdIns3P activity. |
| Protein Name | Wd Repeat Domain Phosphoinositide-Interacting Protein 4Wipi-4Wd Repeat-Containing Protein 45 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Preautophagosomal StructureCytoplasmDiffusely Localized In The Cytoplasm Under Nutrient-Rich ConditionsLocalizes To Autophagic Structures During Starvation-Induced Autophagy |
| Alternative Antibody Names | Anti-Wd Repeat Domain Phosphoinositide-Interacting Protein 4 antibodyAnti-Wipi-4 antibodyAnti-Wd Repeat-Containing Protein 45 antibodyAnti-WDR45 antibodyAnti-WDRX1 antibodyAnti-WDRXI4 antibodyAnti-WIPI4 antibodyAnti-JM5 antibody |
Information sourced from Uniprot.org