| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WDR45 (35-148) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
WDR45 |
| Gene ID: |
11152 |
| Uniprot ID: |
WIPI4_HUMAN |
| Immunogen Region: |
35-148 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 35-148 of human WDR45 (NP_001025067.1). |
| Immunogen Sequence: |
EPLMEKGHLDHEQVGSMGLV EMLHRSNLLALVGGGSSPKF SEISVLIWDDAREGKDSKEK LVLEFTFTKPVLSVRMRHDK IVIVLKNRIYVYSFPDNPRK LFEFDTRDNPKGLC |
| Tissue Specificity | Ubiquitously expressed, with high expression in skeletal muscle and heart. Weakly expressed in liver and placenta. Expression is down-regulated in pancreatic and in kidney tumors. |
| Function | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Together with WIPI1, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Probably recruited to membranes through its PtdIns3P activity. |
| Protein Name | Wd Repeat Domain Phosphoinositide-Interacting Protein 4Wipi-4Wd Repeat-Containing Protein 45 |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Preautophagosomal StructureCytoplasmDiffusely Localized In The Cytoplasm Under Nutrient-Rich ConditionsLocalizes To Autophagic Structures During Starvation-Induced Autophagy |
| Alternative Antibody Names | Anti-Wd Repeat Domain Phosphoinositide-Interacting Protein 4 antibodyAnti-Wipi-4 antibodyAnti-Wd Repeat-Containing Protein 45 antibodyAnti-WDR45 antibodyAnti-WDRX1 antibodyAnti-WDRXI4 antibodyAnti-WIPI4 antibodyAnti-JM5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
