| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Wdr33 |
| Gene ID: | 74320 |
| Uniprot ID: | WDR33_MOUSE |
| Immunogen Region: | 1-208 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of mouse WDR33 (NP_001164437.1). |
| Immunogen Sequence: | MATEIGSPPRFFHMPRFQHQ APRQLFYKRPDFAQQQAMQQ LTFDGKRMRKAVNRKTIDYN PSVIKYLENRIWQRDQRDMR AIQPDAGYYNDLVPPIGMLN NPMNAVTTKFVRTSTNKVKC PVFVVRWTPEGRRLVTGASS GEFTLWNGLTFNFETILQAH DSPVRAMTWSHNDMWMLTAD HGGYVKYWQSNMNNVKMFQA HKEAIREA |
| Tissue Specificity | Most highly expressed in testis. |
| Function | Essential for both cleavage and polyadenylation of pre-mRNA 3' ends. |
| Protein Name | Pre-Mrna 3' End Processing Protein Wdr33Wd Repeat-Containing Protein 33Wd Repeat-Containing Protein Of 146 Kda |
| Database Links | Reactome: R-MMU-159231Reactome: -MMU-72187Reactome: -MMU-72203Reactome: -MMU-73856Reactome: -MMU-77595 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pre-Mrna 3' End Processing Protein Wdr33 antibodyAnti-Wd Repeat-Containing Protein 33 antibodyAnti-Wd Repeat-Containing Protein Of 146 Kda antibodyAnti-Wdr33 antibodyAnti-Wdc146 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89145/357595/STJ11100075_1__06892.1713121734.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYL6B knockout (KO) 293T cells, using [KO Validated] MYL6B Rabbit polyclonal antibody (STJ11100075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and FADD knockout (KO) HeLa cells, using [KO Validated] FADD Rabbit polyclonal antibody (STJ11100017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89090/357549/STJ11100017_1__19285.1713121669.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and FADD knockout (KO) HeLa cells, using [KO Validated] FADD Rabbit polyclonal antibody (STJ11100017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
