| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VPS35 (190-240 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Vps35 |
| Gene ID: |
65114 |
| Uniprot ID: |
VPS35_MOUSE |
| Immunogen Region: |
190-240 aa |
| Specificity: |
Specific for VPS35. |
| Immunogen: |
A synthetic peptide from aa region 190-240 of human VPS35 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse. |
| Function | Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35.however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2 and mediates the association of the CSC with the WASH complex. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 35Maternal-Embryonic 3Vesicle Protein Sorting 35 |
| Database Links | Reactome: R-MMU-3238698 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinEndosomeEarly EndosomeLate Endosome |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 35 antibodyAnti-Maternal-Embryonic 3 antibodyAnti-Vesicle Protein Sorting 35 antibodyAnti-Vps35 antibodyAnti-Mem3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
