| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VPS33B (348-617) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VPS33B |
| Gene ID: |
26276 |
| Uniprot ID: |
VP33B_HUMAN |
| Immunogen Region: |
348-617 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 348-617 of human VPS33B (NP_061138.3). |
| Immunogen Sequence: |
CESIMKKKTKQDFQELIKTE HALLEGFNIRESTSYIEEHI DRQVSPIESLRLMCLLSITE NGLIPKDYRSLKTQYLQSYG PEHLLTFSNLRRAGLLTEQA PGDTLTAVESKVSKLVTDKA AGKITDAFSSLAKRSNFRAI SKKLNLIPRVDGEYDLKVPR DMAYVFGGAYVPLSCRIIEQ VLERRSWQGLDEVVRLLNCS DFAFTDMTKEDKASSESLRL ILVVFLGGCTFSEISALRF |
| Tissue Specificity | Ubiquitous.highly expressed in testis and low expression in the lung. |
| Post Translational Modifications | Phosphorylated on tyrosine residues. (Microbial infection) Dephosphorylated by M.tuberculosis PtpA, which induces the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages. |
| Function | May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 33bHvps33b |
| Database Links | Reactome: R-HSA-9636383Reactome: R-HSA-9754560 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideLysosome MembraneEarly EndosomeCytoplasmic VesicleClathrin-Coated VesicleRecycling EndosomeColocalizes In Clusters With Vipas39 At Cytoplasmic OrganellesColocalizes With Rab11a And Vipas39 On Recycling EndosomesColocalizes With Ap-3ClathrinRab5 And Rab7bColocalizes With MTuberculosis Ptpa In The Cytosol Of Tuberculosis-Infected Macrophages And Associates With Phagosomes |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 33b antibodyAnti-Hvps33b antibodyAnti-VPS33B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
