• STJ70499 staining (0. 1µg/ml) of Human Spleen lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.

Anti-VPS29 antibody (C-Term) (STJ70499)

SKU:
STJ70499-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-VPS29 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: VPS29
Gene ID: 51699
Uniprot ID: VPS29_HUMAN
Immunogen Region: C-Term
Accession Number: NP_057310.1; NP_476528.1
Specificity: This antibody is expected to recognise both human isoforms (represented by NP_057310.1; NP_476528.1).
Immunogen Sequence: DDVKVERIEYKKP
Function Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Acts also as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). In the endosomes, retriever complex drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. Involved in GLUT1 endosome-to-plasma membrane trafficking.the function is dependent of association with ANKRD27. (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.
Protein Name Vacuolar Protein Sorting-Associated Protein 29
Hvps29
Pep11 Homolog
Vesicle Protein Sorting 29
Database Links Reactome: R-HSA-3238698
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Endosome Membrane
Early Endosome
Late Endosome
Alternative Antibody Names Anti-Vacuolar Protein Sorting-Associated Protein 29 antibody
Anti-Hvps29 antibody
Anti-Pep11 Homolog antibody
Anti-Vesicle Protein Sorting 29 antibody
Anti-VPS29 antibody
Anti-DC15 antibody
Anti-DC7 antibody
Anti-MDS007 antibody

Information sourced from Uniprot.org

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