• Western blot analysis of extracts of various cell lines, using VPS28 antibody (STJ111568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using VPS28 Rabbit polyclonal antibody (STJ111568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VPS28 antibody (1-221) (STJ111568)

SKU:
STJ111568

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VPS28 (1-221) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VPS28
Gene ID: 51160
Uniprot ID: VPS28_HUMAN
Immunogen Region: 1-221
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human VPS28 (NP_057292.1).
Immunogen Sequence: MFHGIPATPGIGAPGNKPEL YEEVKLYKNAREREKYDNMA ELFAVVKTMQALEKAYIKDC VSPSEYTAACSRLLVQYKAA FRQVQGSEISSIDEFCRKFR LDCPLAMERIKEDRPITIKD DKGNLNRCIADVVSLFITVM DKLRLEIRAMDEIQPDLREL METMHRMSHLPPDFEGRQTV SQWLQTLSGMSASDELDDSQ VRQMLFDLESAYNAFNRFLH A
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
Protein Name Vacuolar Protein Sorting-Associated Protein 28 Homolog
H-Vps28
Escrt-I Complex Subunit Vps28
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-174490
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Cellular Localisation Cell Membrane
Late Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Vacuolar Protein Sorting-Associated Protein 28 Homolog antibody
Anti-H-Vps28 antibody
Anti-Escrt-I Complex Subunit Vps28 antibody
Anti-VPS28 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance