Anti-VPS25 antibody (Internal) (STJ70843)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-VPS25 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.I |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | VPS25 |
| Gene ID | 84313 |
| Uniprot ID | VPS25_HUMAN |
| Accession Number | NP_115729.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | QPNVDTRQKQ |
Additional Info
| Function | Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. |
| Protein Name | Vacuolar Protein-Sorting-Associated Protein 25Hvps25Dermal Papilla-Derived Protein 9Ell-Associated Protein Of 20 KdaEscrt-Ii Complex Subunit Vps25 |
| Database Links | Reactome: R-HSA-917729Reactome: R-HSA-9610379 |
| Cellular Localisation | CytoplasmEndosome MembraneNucleusNucleoplasmDistributes Diffusely Throughout The Cytoplasm And NucleoplasmBut Exhibits A Punctate Distribution On Coexpression With Chmp6 |
| Alternative Antibody Names | Anti-Vacuolar Protein-Sorting-Associated Protein 25 antibodyAnti-Hvps25 antibodyAnti-Dermal Papilla-Derived Protein 9 antibodyAnti-Ell-Associated Protein Of 20 Kda antibodyAnti-Escrt-Ii Complex Subunit Vps25 antibodyAnti-VPS25 antibodyAnti-DERP9 antibodyAnti-EAP20 antibody |
Information sourced from Uniprot.org