Anti-VPS16 antibody (540-839) (STJ118333)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal VPS16 (540-839) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VPS16 |
| Gene ID | 64601 |
| Uniprot ID | VPS16_HUMAN |
| Immunogen Region | 540-839 |
| Immunogen Sequence | EYEPRSGEQVPLLLKMKRSK LALSKAIESGDTDLVFTVLL HLKNELNRGDFFMTLRNQPM ALSLYRQFCKHQELETLKDL YNQDDNHQELGSFHIRASYA AEERIEGRVAALQTAADAFY KAKNEFAAKATEDQMRLLRL QRRLEDELGGQFLDLSLHDT VTTLILGGHNKRAEQLARDF RIPDKRLWWLKLTALADLED WEELEKFSKSKKSPIGYLPF VEICMKQHNKYEAKKYASR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 540-839 of human VPS16 (NP_072097.2). |
Additional Info
| Tissue Specificity | Ubiquitous. |
| Function | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for recruitment of VPS33A to the HOPS complex. Required for fusion of endosomes and autophagosomes with lysosomes.the function is dependent on its association with VPS33A but not VPS33B. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 16 HomologHvps16 |
| Database Links | Reactome: R-HSA-9754560 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideLysosome MembraneEarly EndosomeCytoplasmic VesicleClathrin-Coated VesicleAutophagosomeColocalizes With Ap-3ClathrinRab5 And Rab7bCytoplasmicPeripheral Membrane Protein Associated With Early Endosomes And Late Endosomes/Lysosomes |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 16 Homolog antibodyAnti-Hvps16 antibodyAnti-VPS16 antibody |
Information sourced from Uniprot.org