Western blot analysis of extracts of rat spinal cord, using VIP antibody (STJ114405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-VIP antibody (31-170) (STJ114405)

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STJ114405
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VIP
Gene ID: 7432
Uniprot ID: VIP_HUMAN
Immunogen Region: 31-170
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 31-170 of human VIP (NP_003372.1).
Immunogen Sequence: APSALRLGDRIPFEGANEPD QVSLKEDIDMLQNALAENDT PYYDVSRNARHADGVFTSDF SKLLGQLSAKKYLESLMGKR VSSNISEDPVPVKRHSDAVF TDNYTRLRKQMAVKKYLNSI LNGKRSSEGESPDFPEELEK
Function Vasoactive intestinal peptide: VIP is a neuropeptide involved in a diverse array of physiological processes through activating the PACAP subfamily of class B1 G protein-coupled receptors: VIP receptor 1 (VPR1) and VIP receptor 2 (VPR2). Abundantly expressed throughout the CNS and peripheral nervous systems where they primarily exert neuroprotective and immune modulatory roles. Also causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM-27 and PHV-42 are two bioactive forms from proteolysis of the same precursor protein, that cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin.
Protein Name Vip Peptides Cleaved Into - Intestinal Peptide Phv-42
Peptide Histidine Valine 42
Phv-42 - Intestinal Peptide Phm-27
Peptide Histidine Methioninamide 27
Phm-27 - Vasoactive Intestinal Peptide
Vip
Vasoactive Intestinal Polypeptide
Database Links Reactome: R-HSA-418555
Reactome: R-HSA-420092
Cellular Localisation Secreted
Alternative Antibody Names Anti-Vip Peptides Cleaved Into - Intestinal Peptide Phv-42 antibody
Anti-Peptide Histidine Valine 42 antibody
Anti-Phv-42 - Intestinal Peptide Phm-27 antibody
Anti-Peptide Histidine Methioninamide 27 antibody
Anti-Phm-27 - Vasoactive Intestinal Peptide antibody
Anti-Vip antibody
Anti-Vasoactive Intestinal Polypeptide antibody
Anti-VIP antibody

Information sourced from Uniprot.org

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