| Host: | Mouse |
| Applications: | FC |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Vinculin for use in FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | 4D4 |
| Conjugation: | FITC |
| Isotype: | IgG1 |
| Formulation: | PBS with 0.02% sodium azide and 0.1% gelatin, pH7.4 |
| Purification: | Protein A/G Purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | FCM 0.2 Mu g per 10^6 cells in a 100 Mu L suspension |
| Storage Instruction: | Store between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
| Gene Symbol: | VCL |
| Gene ID: | 7414 |
| Uniprot ID: | VINC_HUMAN |
| Immunogen: | Recombinant Human Vinculin protein expressed by E.coli |
| Tissue Specificity | Metavinculin is muscle-specific. |
| Post Translational Modifications | Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid. |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. |
| Protein Name | VinculinMetavinculinMv |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-445355Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9725370Reactome: R-HSA-9856530Reactome: R-HSA-9860927 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionAdherens JunctionFocal AdhesionCytoplasmCytoskeletonSarcolemmaCell ProjectionPodosomeRecruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent MannerInteraction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions |
| Alternative Antibody Names | Anti-Vinculin antibodyAnti-Metavinculin antibodyAnti-Mv antibodyAnti-VCL antibody |
Information sourced from Uniprot.org