Anti-Vimentin antibody [SM0448] (STJA0010448)

SKU:
STJA0010448

£170.50 - £233.50
Shipping:
Free Shipping
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Vimentin is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM0448
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H, M, R) 1:1000-1:2000
IHC/IF (M, R) 1:500-1:1000/1:500-1:1000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Vim
Gene ID: 22352
Uniprot ID: VIME_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse vimentin
Post Translational Modifications Phosphorylation by PKN1 inhibits the formation of filaments. Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Protein Name Vimentin
Database Links Reactome: R-MMU-264870
Reactome: -MMU-390522
Reactome: -MMU-9013422
Reactome: -MMU-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus Matrix
Cell Membrane
Alternative Antibody Names Anti-Vimentin antibody
Anti-Vim antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance