• Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Vimentin in paraffin-embedded rat uterus using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Vimentin in paraffin-embedded rat testis using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Vimentin in paraffin-embedded mouse brain using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Vimentin in paraffin-embedded human liver cancer using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Vimentin in paraffin-embedded human colon using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using Vimentin Rabbit polyclonal antibody (STJ11104460) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VIM antibody (10-101) (STJ11104460)

SKU:
STJ11104460

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Vimentin (10-101) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VIM
Gene ID: 7431
Uniprot ID: VIME_HUMAN
Immunogen Region: 10-101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-101 of human Vimentin (NP_003371.2).
Immunogen Sequence: SYRRMFGGPGTASRPSSSRS YVTTSTRTYSLGSALRPSTS RSLYASSPGGVYATRSSAVR LRSSVPGVRLLQDSVDFSLA DAINTEFKNTRT
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post Translational Modifications Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Protein Name Vimentin
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-390522
Reactome: R-HSA-6785807
Reactome: R-HSA-9013422
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus Matrix
Cell Membrane
Alternative Antibody Names Anti-Vimentin antibody
Anti-VIM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance