• Immunofluorescence analysis of L929 cells using VILL Rabbit polyclonal antibody (STJ117635) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using VILL Rabbit polyclonal antibody (STJ117635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-VILL antibody (637-856) (STJ117635)

SKU:
STJ117635

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:200-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VILL
Gene ID: 50853
Uniprot ID: VILL_HUMAN
Immunogen Region: 637-856
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 637-856 of human VILL (NP_056957.3).
Immunogen Sequence: LDKYDIMLLDTWQEIFLWLG EAASEWKEAVAWGQEYLKTH PAGRSPATPIVLVKQGHEPP TFIGWFFTWDPYKWTSHPSH KEVVDGSPAAASTISEITAE VNNLRLSRWPGNGRAGAVAL QALKGSQDSSENDLVRSPKS AGSRTSSSVSSTSATINGGL RREQLMHQAVEDLPEGVDPA RREFYLSDSDFQDIFGKSKE EFYSMATWRQRQEKKQLGFF
Tissue Specificity Ubiquitously expressed in 16 tissues examined.
Function Possible tumor suppressor.
Protein Name Villin-Like Protein
Alternative Antibody Names Anti-Villin-Like Protein antibody
Anti-VILL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance