Anti-VHL, Biotinylated antibody (Internal) {Biotin} (STJ73173)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationBiotin
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-VHL, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 17kDa band observed in Human Ovary lysates (calculated MW of 19.7kDa according to NP_937799.1). See non-biotinylated parental product's datasheet for further QC data. Recommend |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | VHL |
| Gene ID | 7428 |
| Uniprot ID | VHL_HUMAN |
| Accession Number | NP_000542.1; NP_937799.1; XP_011532380.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | RSLVKPENYRRLD. |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1). |
Additional Info
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor SuppressorProtein G7Pvhl |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-3232142Reactome: R-HSA-8951664Reactome: R-HSA-9682706Reactome: R-HSA-9694686Reactome: R-HSA-9706019Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: CytoplasmCell MembranePeripheral Membrane ProteinEndoplasmic ReticulumNucleusFound Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-AssociatedColocalizes With Adrb2 At The Cell MembraneIsoform 3: CytoplasmEqually Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative Antibody Names | Anti-Von Hippel-Lindau Disease Tumor Suppressor antibodyAnti-Protein G7 antibodyAnti-Pvhl antibodyAnti-VHL antibody |
Information sourced from Uniprot.org