• Human Kidney tissue was stained with Anti-pVHL (ABT-PVHL) Antibody
  • Human liver tissue was stained with Anti-pVHL (ABT-PVHL) Antibody
  • Human pancreas tissue was stained with Anti-pVHL (ABT-PVHL) Antibody
  • Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-pVHL (ABT-PVHL) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: RajiPredicted band size: 24kDaObserved band size: 17kDa
  • Immunohistochemical analysis of paraffin-embedded kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded kidney. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Liver. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-VHL antibody (150-213 aa) [ABT-PVHL] (STJ196864)

SKU:
STJ196864

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-von Hippel-Lindau disease tumor suppressor (150-213 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT-PVHL
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 500-2000
IHC-P 1:100-500
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VHL
Gene ID: 7428
Uniprot ID: VHL_HUMAN
Immunogen Region: 150-213 aa
Specificity: This antibody detects endogenous levels of human pVHL. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human pVHL at the amino acid range 150-213
Function Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR.
Protein Name Von Hippel-Lindau Disease Tumor Suppressor
Protein G7
Pvhl
Database Links Reactome: R-HSA-1234176
Reactome: R-HSA-3232142
Reactome: R-HSA-8951664
Reactome: R-HSA-9682706
Reactome: R-HSA-9694686
Reactome: R-HSA-9706019
Reactome: R-HSA-983168
Cellular Localisation Isoform 1: Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Endoplasmic Reticulum
Nucleus
Found Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-Associated
Colocalizes With Adrb2 At The Cell Membrane
Isoform 3: Cytoplasm
Equally Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated
Alternative Antibody Names Anti-Von Hippel-Lindau Disease Tumor Suppressor antibody
Anti-Protein G7 antibody
Anti-Pvhl antibody
Anti-VHL antibody

Information sourced from Uniprot.org

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