| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Monkey/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-VHL (130-200) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 µg/µl |
| Dilution Range: | WB: 1:500ELISA: 1:10, 000IP: 1:200IHC: 1:50-1:150ICC: 1:50-1:150 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | VHL |
| Gene ID: | 7428 |
| Uniprot ID: | VHL_HUMAN |
| Immunogen Region: | 130-200 |
| Immunogen: | Synthetic peptide taken within amino acid region 130-200 on human Von Hippel-Lindau disease tumor suppressor protein. Host Rabbit Species Cross Reactivity Human, Monkey, Mouse, Rat |
| Tissue Specificity | Expressed in the adult and fetal brain and kidney. |
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor SuppressorProtein G7Pvhl |
| Database Links | Reactome: R-HSA-1234176Reactome: R-HSA-3232142Reactome: R-HSA-8951664Reactome: R-HSA-9682706Reactome: R-HSA-9694686Reactome: R-HSA-9706019Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: CytoplasmCell MembranePeripheral Membrane ProteinEndoplasmic ReticulumNucleusFound Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-AssociatedColocalizes With Adrb2 At The Cell MembraneIsoform 3: CytoplasmEqually Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative Antibody Names | Anti-Von Hippel-Lindau Disease Tumor Suppressor antibodyAnti-Protein G7 antibodyAnti-Pvhl antibodyAnti-VHL antibody |
Information sourced from Uniprot.org
