| Host: | Rabbit |
| Applications: | IF/WB/IHC/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Vascular endothelial growth factor A, long form is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IF 1:50-200 WB 1:500-2000 IHC 1:50-300 IHC 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | VEGFA |
| Gene ID: | 7422 |
| Uniprot ID: | VEGFA_HUMAN |
| Specificity: | VEGF protein detects endogenous levels of VEGFA |
| Immunogen: | Recombinant Protein of VEGF of VEGFA |
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Protein Name | Vascular Endothelial Growth Factor A - Long Form L-Vegf Vascular Permeability Factor Vpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-HSA-114608 Reactome: R-HSA-1234158 Reactome: R-HSA-194138 Reactome: R-HSA-194313 Reactome: R-HSA-195399 Reactome: R-HSA-4420097 P15692-4 Reactome: R-HSA-5218921 P15692-4 Reactome: R-HSA-6785807 Reactome: R-HSA-8866910 Reactome: R-HSA-9679191 P15692-4 |
| Cellular Localisation | N-Vegf: Cytoplasm Nucleus Cytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic Conditions Vegfa: Secreted Isoform L-Vegf189: Endoplasmic Reticulum Golgi Apparatus Secreted Extracellular Space Extracellular Matrix Isoform Vegf121: Secreted Isoform Vegf165: Secreted Isoform Vegf189: Secreted Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular Matrix Although It May Be Released As A Soluble Form By Heparin Heparinase Or Plasmin |
| Alternative Antibody Names | Anti-Vascular Endothelial Growth Factor A - Long Form antibody Anti-L-Vegf antibody Anti-Vascular Permeability Factor antibody Anti-Vpf Cleaved Into - N-Vegf - Vegfa antibody Anti-VEGFA antibody Anti-VEGF antibody |
Information sourced from Uniprot.org