| Host: |
Goat |
| Applications: |
WB/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Vascular endothelial growth factor A is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:2000IF 1:50-1:2501.Martinho JM Coelho A Oliveiros B et al. Int Endod J 2021 May PMID: 339994332.Oliveira SF MSc Thesis University of Coimbra Portugal 2019Rodrigues T Borges P Mar L et al. Pharmacol Res. 2020 Sep. PMID: |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
VEGFA |
| Gene ID: |
7422 |
| Uniprot ID: |
VEGFA_HUMAN |
| Accession Number: |
ENSG00000112715 |
| Specificity: |
Detects endogenous levels of total VEGFA by Western blot in whole cell and tissue lysates. |
| Immunogen: |
Purified recombinant human VEGFA isoform 6 produced in E. coli. |
| Immunogen Sequence: |
MNFLLSWVHWSLALLLYLHH AKWSQAAPMAEGGGQNHHEV VKFMDVYQRSYCHPIETLVD IFQEYPDEIEYIFKPSCVPL MRCGGCCNDEGLECVPTEES NITMQIMRIKPHQGQHIGEM SFLQHNKCECRPKKDRARQE KCDKPRR |
| Tissue Specificity | Higher expression in pituitary tumors than the pituitary gland. Isoform VEGF189: Widely expressed. Isoform VEGF165: Widely expressed. Isoform VEGF121: Widely expressed. Isoform VEGF206: Not widely expressed. Isoform VEGF145: Not widely expressed. |
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1234158Reactome: R-HSA-194138Reactome: R-HSA-194313Reactome: R-HSA-195399Reactome: R-HSA-4420097 P15692-4Reactome: R-HSA-5218921 P15692-4Reactome: R-HSA-6785807Reactome: R-HSA-8866910Reactome: R-HSA-9679191 P15692-4 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf189: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf121: SecretedIsoform Vegf165: SecretedIsoform Vegf189: SecretedCell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative Antibody Names | Anti-Vascular Endothelial Growth Factor A - Long Form antibodyAnti-L-Vegf antibodyAnti-Vascular Permeability Factor antibodyAnti-Vpf Cleaved Into - N-Vegf - Vegfa antibodyAnti-VEGFA antibodyAnti-VEGF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
