• STJ73742 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73742 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-VE-cadherin antibody (Internal) (STJ73742)

SKU:
STJ73742-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-VE-cadherin (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of HeLa cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CDH5
Gene ID: 1003
Uniprot ID: CADH5_HUMAN
Immunogen Region: Internal
Accession Number: NP_001786.2
Immunogen Sequence: KQARAHGKSVPE
Post Translational Modifications Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. O-glycosylated.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Protein Name Cadherin-5
7b4 Antigen
Vascular Endothelial Cadherin
Ve-Cadherin
Cd Antigen Cd144
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-5218920
Cellular Localisation Cell Junction
Cell Membrane
Single-Pass Type I Membrane Protein
Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries
Krit1 And Cdh5 Reciprocally Regulate Their Localization To Endothelial Cell-Cell Junctions
Alternative Antibody Names Anti-Cadherin-5 antibody
Anti-7b4 Antigen antibody
Anti-Vascular Endothelial Cadherin antibody
Anti-Ve-Cadherin antibody
Anti-Cd Antigen Cd144 antibody
Anti-CDH5 antibody

Information sourced from Uniprot.org

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