• Immunofluorescence analysis of HeLa cells using VDAC3 Rabbit polyclonal antibody (STJ112562) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of VDAC3 in paraffin-embedded mouse lung using VDAC3 Rabbit polyclonal antibody (STJ112562) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of VDAC3 in paraffin-embedded mouse kidney using VDAC3 Rabbit polyclonal antibody (STJ112562) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse heart, using VDAC3 Rabbit polyclonal antibody (STJ112562) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-VDAC3 antibody (1-283) (STJ112562)

SKU:
STJ112562

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VDAC3
Gene ID: 7419
Uniprot ID: VDAC3_HUMAN
Immunogen Region: 1-283
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-283 of human VDAC3 (NP_005653.3).
Immunogen Sequence: MCNTPTYCDLGKAAKDVFNK GYGFGMVKIDLKTKSCSGVE FSTSGHAYTDTGKASGNLET KYKVCNYGLTFTQKWNTDNT LGTEISWENKLAEGLKLTLD TIFVPNTGKKSGKLKASYKR DCFSVGSNVDIDFSGPTIYG WAVLAFEGWLAGYQMSFDTA KSKLSQNNFALGYKAADFQL HTHVNDGTEFGGSIYQKVNE KIETSINLAWTAGSNNTRFG IAAKYMLDCRTSLSAKVNN
Tissue Specificity Expressed in erythrocytes (at protein level). Widely expressed. Highest in testis.
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. Involved in male fertility and sperm mitochondrial sheath formation.
Protein Name Voltage-Dependent Anion-Selective Channel Protein 3
Vdac-3
Hvdac3
Outer Mitochondrial Membrane Protein Porin 3
Database Links Reactome: R-HSA-5205685
Reactome: R-HSA-5689880
Reactome: R-HSA-8949215
Cellular Localisation Mitochondrion Outer Membrane
Membrane
May Localize To Non-Mitochondrial Membranes
Alternative Antibody Names Anti-Voltage-Dependent Anion-Selective Channel Protein 3 antibody
Anti-Vdac-3 antibody
Anti-Hvdac3 antibody
Anti-Outer Mitochondrial Membrane Protein Porin 3 antibody
Anti-VDAC3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance