• Immunofluorescence analysis of PC-12 cells using VDAC2 Rabbit polyclonal antibody (STJ11101478) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using VDAC2 Rabbit polyclonal antibody (STJ11101478) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using VDAC2 Rabbit polyclonal antibody (STJ11101478) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using VDAC2 Rabbit polyclonal antibody (STJ11101478) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using VDAC2 Rabbit polyclonal antibody (STJ11101478) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain using VDAC2 Rabbit polyclonal antibody (STJ11101478) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VDAC2 antibody (STJ11101478) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-VDAC2 antibody (14-24) (STJ11101478)

SKU:
STJ11101478

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VDAC2 (14-24) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VDAC2
Gene ID: 7417
Uniprot ID: VDAC2_HUMAN
Immunogen Region: 14-24
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 14-24 of human VDAC2 (NP_003366.2).
Immunogen Sequence: DIFNKGFGFGL
Tissue Specificity Expressed in erythrocytes (at protein level). Expressed in all tissues examined.
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol. Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway.
Protein Name Voltage-Dependent Anion-Selective Channel Protein 2
Vdac-2
Hvdac2
Outer Mitochondrial Membrane Protein Porin 2
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-8949215
Cellular Localisation Mitochondrion Outer Membrane
Membrane
May Localize To Non-Mitochondrial Membranes
Alternative Antibody Names Anti-Voltage-Dependent Anion-Selective Channel Protein 2 antibody
Anti-Vdac-2 antibody
Anti-Hvdac2 antibody
Anti-Outer Mitochondrial Membrane Protein Porin 2 antibody
Anti-VDAC2 antibody

Information sourced from Uniprot.org

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