| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VCP (647-806) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VCP |
| Gene ID: |
7415 |
| Uniprot ID: |
TERA_HUMAN |
| Immunogen Region: |
647-806 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1). |
| Immunogen Sequence: |
LPDEKSRVAILKANLRKSPV AKDVDLEFLAKMTNGFSGAD LTEICQRACKLAIRESIESE IRRERERQTNPSAMEVEEDD PVPEIRRDHFEEAMRFARRS VSDNDIRKYEMFAQTLQQSR GFGSFRFPSGNQGGAGPSQG SGGGTGGSVYTEDNDDDLYG |
| Post Translational Modifications | Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation. Phosphorylated in mitotic cells. ISGylated. Methylation at Lys-315 catalyzed by VCPKMT is increased in the presence of ASPSCR1. Lys-315 methylation may decrease ATPase activity. |
| Function | Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Involved in clearance process by mediating G3BP1 extraction from stress granules. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Together with SPRTN metalloprotease, involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis. Involved in interstrand cross-link repair in response to replication stress by mediating unloading of the ubiquitinated CMG helicase complex. Mediates extraction of PARP1 trapped to chromatin: recognizes and binds ubiquitinated PARP1 and promotes its removal. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with RIGI: interaction takes place when RIGI is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of RIGI. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. |
| Protein Name | Transitional Endoplasmic Reticulum AtpaseTer Atpase15s Mg(2+-Atpase P97 SubunitValosin-Containing ProteinVcp |
| Database Links | Reactome: R-HSA-110320Reactome: R-HSA-3371511Reactome: R-HSA-382556Reactome: R-HSA-532668Reactome: R-HSA-5358346Reactome: R-HSA-5362768Reactome: R-HSA-5678895Reactome: R-HSA-5689877Reactome: R-HSA-5689896Reactome: R-HSA-6798695Reactome: R-HSA-8866654Reactome: R-HSA-8876725Reactome: R-HSA-8951664Reactome: R-HSA-9013407Reactome: R-HSA-9646399Reactome: R-HSA-9678110Reactome: R-HSA-9694614Reactome: R-HSA-9755511 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic ReticulumNucleusStress GranulePresent In The Neuronal Hyaline Inclusion Bodies Specifically Found In Motor Neurons From Amyotrophic Lateral Sclerosis PatientsPresent In The Lewy Bodies Specifically Found In Neurons From Parkinson Disease PatientsRecruited To The Cytoplasmic Surface Of The Endoplasmic Reticulum Via Interaction With Amfr/Gp78Following Dna Double-Strand BreaksRecruited To The Sites Of DamageRecruited To Stalled Replication Forks Via Interaction With SprtnRecruited To Damaged Lysosomes Decorated With K48-Linked Ubiquitin ChainsColocalizes With Tia1Zfand1 And G3bp1 In Cytoplasmic Stress Granules (Sgs) In Response To Arsenite-Induced Stress Treatment |
| Alternative Antibody Names | Anti-Transitional Endoplasmic Reticulum Atpase antibodyAnti-Ter Atpase antibodyAnti-15s Mg(2+-Atpase P97 Subunit antibodyAnti-Valosin-Containing Protein antibodyAnti-Vcp antibodyAnti-VCP antibodyAnti-HEL-220 antibodyAnti-HEL-S-70 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
