![Immunofluorescence analysis of L929 cells using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98168/381871/STJ119297_1__17738.1713150101.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and VAT1 knockout (KO) 293T cells, using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98168/381872/STJ119297_2__48752.1713150102.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and VAT1 knockout (KO) 293T cells, using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98168/381873/STJ119297_3__83953.1713150103.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![Immunofluorescence analysis of L929 cells using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98168/381871/STJ119297_1__17738.1713150101.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] VAT1 Rabbit polyclonal antibody (STJ119297) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-VAT1 antibody (200-300) (STJ119297)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal VAT1 (200-300) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VAT1 |
| Gene ID | 10493 |
| Uniprot ID | VAT1_HUMAN |
| Immunogen Region | 200-300 |
| Immunogen Sequence | GVGMAAVQLCRTVENVTVFG TASASKHEALKENGVTHPID YHTTDYVDEIKKISPKGVDI VMDPLGGSDTAKGYNLLKPM GKVVTYGMANLLTGPKRNLM A |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human VAT1 (NP_006364.2). |
Additional Info
| Tissue Specificity | Expressed in brain. Also expressed in glioblastoma cells. |
| Function | Possesses ATPase activity. Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2). |
| Protein Name | Synaptic Vesicle Membrane Protein Vat-1 Homolog |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmMitochondrion Outer MembranePeripheral Membrane ProteinThe Majority Is Localized In The Cytoplasm And A Small Amount Is Associated With Mitochondria |
| Alternative Antibody Names | Anti-Synaptic Vesicle Membrane Protein Vat-1 Homolog antibodyAnti-VAT1 antibody |
Information sourced from Uniprot.org