• Immunohistochemistry analysis of paraffin-embedded human kidney cancer using VANGL2 antibody (STJ110135) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using VANGL2 antibody (STJ110135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-VANGL2 antibody (1-110) (STJ110135)

SKU:
STJ110135

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VANGL2
Gene ID: 57216
Uniprot ID: VANG2_HUMAN
Immunogen Region: 1-110
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human VANGL2 (NP_065068.1).
Immunogen Sequence: MDTESQYSGYSYKSGHSRSS RKHRDRRDRHRSKSRDGGRG DKSVTIQAPGEPLLDNESTR GDERDDNWGETTTVVTGTSE HSISHDDLTRIAKDMEDSVP LDCSRHLGVA
Function Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear.
Protein Name Vang-Like Protein 2
Loop-Tail Protein 1 Homolog
Strabismus 1
Van Gogh-Like Protein 2
Database Links Reactome: R-HSA-4086400
Reactome: R-HSA-4608870
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Reactome: R-HSA-9696273
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Vang-Like Protein 2 antibody
Anti-Loop-Tail Protein 1 Homolog antibody
Anti-Strabismus 1 antibody
Anti-Van Gogh-Like Protein 2 antibody
Anti-VANGL2 antibody
Anti-KIAA1215 antibody
Anti-STB1 antibody

Information sourced from Uniprot.org

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