• Western blot analysis of extracts of various cell lines, using VAMP1 antibody (STJ111459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat spleen using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human gastric cancer using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using VAMP1 antibody (STJ111459) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using VAMP1 antibody (STJ111459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-VAMP1 antibody (1-96) (STJ111459)

SKU:
STJ111459

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VAMP1 (1-96) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP1
Gene ID: 6843
Uniprot ID: VAMP1_HUMAN
Immunogen Region: 1-96
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-96 of human VAMP1 (NP_954740.1).
Immunogen Sequence: MSAPAQPPAEGTEGTAPGGG PPGPPPNMTSNRRLQQTQAQ VEEVVDIIRVNVDKVLERDQ KLSELDDRADALQAGASQFE SSAAKLKRKYWWKNCK
Tissue Specificity Nervous system, skeletal muscle and adipose tissue.
Post Translational Modifications (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.
Function Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Protein Name Vesicle-Associated Membrane Protein 1
Vamp-1
Synaptobrevin-1
Database Links Reactome: R-HSA-5250955
Reactome: R-HSA-5250981
Reactome: R-HSA-5250989
Cellular Localisation Isoform 1: Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Single-Pass Type Iv Membrane Protein
Synapse
Synaptosome
Isoform 2: Cytoplasmic Vesicle Membrane
Isoform 3: Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 1 antibody
Anti-Vamp-1 antibody
Anti-Synaptobrevin-1 antibody
Anti-VAMP1 antibody
Anti-SYB1 antibody

Information sourced from Uniprot.org

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