• IP antibody use：5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 , 1. untransfected 293 cell lysate , 2. transfected 293 cell lysate with V5-tag fusion protein, 3. IP (untransfted 293+anti-V5 mAb+Protein G agarose) , 4. IP (transfected 293+ normal Mouse IgG+Protein G agarose) , 5. IP (transfected 293+anti-V5 mAb+ Protein G agarose) , 6. IP (transfected 293+Protein G) , 7. Recombinant protein (E.coli)
  • IF analysis of 293 cells transfected with a V5-tag protein, 1:2000 dilution (blue DAPI, red anti-V5)

Anti-V5-Tag antibody [3C8] (STJ96907)

SKU:
STJ96907

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IP/IF
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-V5-Tag-Tag is suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 3C8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:5000
IP 1:200
IF 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Specificity: The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
Immunogen: Synthetic Peptide of V5-Tag
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance