• Western blot detection of HAUSP/USP7 in Hela and K562 cell lysates using HAUSP/USP7 mouse mAb (1:200 diluted) , with super ECL.Predicted band size:135KDa.Observed band size:135KDa.

Anti-USP7 antibody [3E4-D10-E8] (STJ99076)

SKU:
STJ99076

Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Ubiquitin carboxyl-terminal hydrolase 7 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: 3E4-D10-E8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP7
Gene ID: 7874
Uniprot ID: UBP7_HUMAN
Specificity: This antibody detects endogenous levels of HAUSP/USP7 and does not cross-react with related proteins.
Immunogen: Purified recombinant human HAUSP/USP7 protein fragments expressed in E.coli.
Post Translational Modifications Isoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated. Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated. Isoform 1 and isoform 2: Not sumoylated. Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110.leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
Function Hydrolase that deubiquitinates target proteins such as FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Deubiquitinates KMT2E/MLL5 preventing KMT2E/MLL5 proteasomal-mediated degradation. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6. Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex.may act by deubiquitinating components of the PRC1-like complex. Able to mediate deubiquitination of histone H2B.it is however unsure whether this activity takes place in vivo. Exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function. Plays a role in the maintenance of the circadian clock periodicity via deubiquitination and stabilization of the CRY1 and CRY2 proteins. Deubiquitinates REST, thereby stabilizing REST and promoting the maintenance of neural progenitor cells. Deubiquitinates SIRT7, inhibiting SIRT7 histone deacetylase activity and regulating gluconeogenesis. Involved in the regulation of WASH-dependent actin polymerization at the surface of endosomes and the regulation of endosomal protein recycling. It maintains optimal WASH complex activity and precise F-actin levels via deubiquitination of TRIM27 and WASHC1. Mediates the deubiquitination of phosphorylated DEPTOR, promoting its stability and leading to decreased mTORC1 signaling. (Microbial infection) Contributes to the overall stabilization and trans-activation capability of the herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 during HSV-1 infection. (Microbial infection) Upon infection with Epstein-Barr virus, the interaction with viral EBNA1 increases the association of USP7 with PML proteins, which is required for the polyubiquitylation and degradation of PML.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 7
Deubiquitinating Enzyme 7
Herpesvirus-Associated Ubiquitin-Specific Protease
Ubiquitin Thioesterase 7
Ubiquitin-Specific-Processing Protease 7
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6804757
Reactome: R-HSA-8866652
Reactome: R-HSA-8948747
Cellular Localisation Nucleus
Cytoplasm
Pml Body
Chromosome
Present In A Minority Of Nd10 Nuclear Bodies
Association With Icp0/Vmw110 At Early Times Of Infection Leads To An Increased Proportion Of Usp7-Containing Nd10
Colocalizes With Atxn1 In The Nucleus
Colocalized With Daxx In Speckled Structures
Colocalized With Pml And Pten In Promyelocytic Leukemia Protein (Pml) Nuclear Bodies
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 7 antibody
Anti-Deubiquitinating Enzyme 7 antibody
Anti-Herpesvirus-Associated Ubiquitin-Specific Protease antibody
Anti-Ubiquitin Thioesterase 7 antibody
Anti-Ubiquitin-Specific-Processing Protease 7 antibody
Anti-USP7 antibody
Anti-HAUSP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance