• Western blot analysis of mouse-kidney lysis using USP44 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of lysates from LOVO and NIH/3T3 cells, using USP44 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293T cells using USP44 Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-USP44 antibody (211-260 aa) (STJ96206)

SKU:
STJ96206

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin carboxyl-terminal hydrolase 44 (211-260 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP44
Gene ID: 84101
Uniprot ID: UBP44_HUMAN
Immunogen Region: 211-260 aa
Specificity: USP44 Polyclonal Antibody detects endogenous levels of USP44 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human USP44 at the amino acid range 211-260
Post Translational Modifications Dephosphorylated by CTDP1. Ubiquitinated.undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome.
Function Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Promotes also the deubiquitination of histone H2A and H2B. Recruited to RNF8/RNF168-ubiquitinated chromatin surrounding double stranded breaks (DSBs), promotes hydrolysis of such ubiquitin conjugates, thus negatively regulating protein recruitment to damaged chromatin. Participates in nucleotide excision repair (NER) pathway by deubiquitinating DDB2 to prevent its premature degradation so it can remain on damaged chromatin. Promotes FOXP3 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability and increased regulatory T-cell lineage stability. Plays also a positive role in innate immune response to DNA viruses by deubiquitinating STING1, selectively removing its 'Lys-48'-linked polyubiquitin chains and stabilizing it.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 44
Deubiquitinating Enzyme 44
Ubiquitin Thioesterase 44
Ubiquitin-Specific-Processing Protease 44
Database Links Reactome: R-HSA-5689880
Cellular Localisation Nucleus
Cytoplasm
Peaks In Interphase
With Relatively Low Levels Maintained Throughout Mitosis
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 44 antibody
Anti-Deubiquitinating Enzyme 44 antibody
Anti-Ubiquitin Thioesterase 44 antibody
Anti-Ubiquitin-Specific-Processing Protease 44 antibody
Anti-USP44 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance