Anti-USP36 antibody (501-550 aa) (STJ96201)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human USP36 at the amino acid range 501-550
General Information
| Short Description | Rabbit polyclonal anti-Ubiquitin carboxyl-terminal hydrolase 36 (501-550 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | USP36 |
| Gene ID | 57602 |
| Uniprot ID | UBP36_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human USP36 at the amino acid range 501-550 |
| Immunogen Region | 501-550 aa |
| Specificity | USP36 Polyclonal Antibody detects endogenous levels of USP36 protein. |
Additional Info
| Post Translational Modifications | Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels. |
| Function | Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability. Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm. Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions. Deubiquitinates SOD2, regulates SOD2 protein stability. Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins. Promotes CEP63 stabilization through 'Lys-48'-linked deubiquitination leading to increased stability. Acts as a SUMO ligase to promote EXOSC10 sumoylation critical for the nucleolar RNA exosome function in rRNA processing. Binds to pre-rRNAs. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 36Deubiquitinating Enzyme 36Ubiquitin Thioesterase 36Ubiquitin-Specific-Processing Protease 36 |
| Database Links | |
| Cellular Localisation | NucleusNucleolusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 36 antibodyAnti-Deubiquitinating Enzyme 36 antibodyAnti-Ubiquitin Thioesterase 36 antibodyAnti-Ubiquitin-Specific-Processing Protease 36 antibodyAnti-USP36 antibodyAnti-KIAA1453 antibody |
Information sourced from Uniprot.org