| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Ubiquitin carboxyl-terminal hydrolase 15 (81-130 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP15 |
| Gene ID: |
9958 |
| Uniprot ID: |
UBP15_HUMAN |
| Immunogen Region: |
81-130 aa |
| Specificity: |
USP15 Polyclonal Antibody detects endogenous levels of USP15 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human USP15 at the amino acid range 81-130 |
| Post Translational Modifications | Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome. Ubiquitinated, leading to degradation by the proteasome. |
| Function | Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery. Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 15Deubiquitinating Enzyme 15Ubiquitin Thioesterase 15Ubiquitin-Specific-Processing Protease 15Unph-2Unph4 |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-5689603Reactome: R-HSA-5689880 |
| Cellular Localisation | CytoplasmNucleusMitochondrion |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 15 antibodyAnti-Deubiquitinating Enzyme 15 antibodyAnti-Ubiquitin Thioesterase 15 antibodyAnti-Ubiquitin-Specific-Processing Protease 15 antibodyAnti-Unph-2 antibodyAnti-Unph4 antibodyAnti-USP15 antibodyAnti-KIAA0529 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
