• Western blot analysis of extracts of Rat spleen, using USF1 antibody (STJ11104102) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using USF1 antibody (STJ11104102) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-USF1 antibody (100-200) [S4102RM] (STJ11104102)

SKU:
STJ11104102

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4102RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USF1
Gene ID: 7391
Uniprot ID: USF1_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human USF1 (P22415).
Immunogen Sequence: TSDDAVDTEGTAAETHYTYF PSTAVGDGAGGTTSGSTAAV VTTQGSEALLGQATPPGTGQ FFVMMSPQEVLQGGSQRSIA PRTHPYSPKSEAPRTTRDEK R
Function Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Protein Name Upstream Stimulatory Factor 1
Class B Basic Helix-Loop-Helix Protein 11
Bhlhb11
Major Late Transcription Factor 1
Database Links Reactome: R-HSA-9018519
Reactome: R-HSA-9824585
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Upstream Stimulatory Factor 1 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 11 antibody
Anti-Bhlhb11 antibody
Anti-Major Late Transcription Factor 1 antibody
Anti-USF1 antibody
Anti-BHLHB11 antibody
Anti-USF antibody

Information sourced from Uniprot.org

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