| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Uromodulin (175-324) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UMOD |
| Gene ID: |
7369 |
| Uniprot ID: |
UROM_HUMAN |
| Immunogen Region: |
175-324 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 175-324 of human Tamm Horsfall Glycoprotein. (NP_003352.2). |
| Immunogen Sequence: |
LRGWYRFVGQGGARMAETCV PVLRCNTAAPMWLNGTHPSS DEGIVSRKACAHWSGHCCLW DASVQVKACAGGYYVYNLTA PPECHLAYCTDPSSVEGTCE ECSIDEDCKSNNGRWHCQCK QDFNITDISLLEHRLECGAN DMKVSLGKCQ |
| Tissue Specificity | Expressed in the tubular cells of the kidney. Most abundant protein in normal urine (at protein level). Synthesized exclusively in the kidney. Expressed exclusively by epithelial cells of the thick ascending limb of Henle's loop (TALH) and of distal convoluted tubule lumen. |
| Post Translational Modifications | N-glycosylated. N-glycan heterogeneity at Asn-232: Hex7HexNAc6 (major) and dHex1Hex7HexNAc6 (minor).at Asn-322: dHex1Hex6HexNAc5 (minor), dHex1Hex7HexNAc6 (major) and dHex1Hex8HexNAc7 (minor).at Asn-396: Hex6HexNAc5 (major), dHex1Hex6HexNAc5 (minor) and Hex7HexNAc6 (minor). Glycosylated Asn-232 interacts with E.coli adhesin fimH. Other complex glycosylation sites may serve as binding sites for proteins from other bacteria inclduding K.pneumoniae, P.aeruginosa and S.mitis. Proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in urine. This cleavage is catalyzed by HPN. |
| Function | Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable). May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia. Uromodulin, secreted form: In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Protects against urinary tract infections by binding to type 1 fimbriated E.coli. Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation. Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens. |
| Protein Name | UromodulinTamm-Horsfall Urinary GlycoproteinThp Cleaved Into - Uromodulin - Secreted Form |
| Database Links | Reactome: R-HSA-446203 |
| Cellular Localisation | Apical Cell MembraneLipid-AnchorGpi-AnchorBasolateral Cell MembraneCell ProjectionCilium MembraneOnly A Small Fraction Sorts To The Basolateral Pole Of Tubular Epithelial Cells Compared To Apical LocalizationSecreted Into Urine After CleavageColocalizes With Nphp1 And Kif3aUromodulinSecreted Form: SecretedDetected In Urine |
| Alternative Antibody Names | Anti-Uromodulin antibodyAnti-Tamm-Horsfall Urinary Glycoprotein antibodyAnti-Thp Cleaved Into - Uromodulin - Secreted Form antibodyAnti-UMOD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
