Anti-Ubiquitin Conjugating Enzyme E2 L3 antibody [R02-4B2] (STJA0035395)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Ube2L3/UBCH7
General Information
| Short Description | Rabbit monoclonal anti-Ubiquitin Conjugating Enzyme E2 L3 for use in WB, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-4B2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | UBE2L3 |
| Gene ID | 7332 |
| Uniprot ID | UB2L3_HUMAN |
| Immunogen | A synthetic peptide of human Ube2L3/UBCH7 |
Additional Info
| Tissue Specificity | Ubiquitous, with highest expression in testis. |
| Post Translational Modifications | Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. Autoubiquitinated in vitro. |
| Function | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine.in contrast, it has activity with the RBR family E3 enzymes, such as PRKN, RNF31 and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates ubiquitination by the CUL9-RBX1 complex. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 L3E2 Ubiquitin-Conjugating Enzyme L3L-UbcUbch7Ubiquitin Carrier Protein L3Ubiquitin-Conjugating Enzyme E2-F1Ubiquitin-Protein Ligase L3 |
| Database Links | Reactome: R-HSA-5205685Reactome: R-HSA-5357905Reactome: R-HSA-5675482Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 L3 antibodyAnti-E2 Ubiquitin-Conjugating Enzyme L3 antibodyAnti-L-Ubc antibodyAnti-Ubch7 antibodyAnti-Ubiquitin Carrier Protein L3 antibodyAnti-Ubiquitin-Conjugating Enzyme E2-F1 antibodyAnti-Ubiquitin-Protein Ligase L3 antibodyAnti-UBE2L3 antibodyAnti-UBCE7 antibodyAnti-UBCH7 antibody |
Information sourced from Uniprot.org