| Host: | Rabbit |
| Applications: | WB/IHC-F/IHC-P/ICC/IF/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Ubiquitin Conjugating Enzyme E2 C for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R08-4F5 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | UBE2C |
| Gene ID: | 11065 |
| Uniprot ID: | UBE2C_HUMAN |
| Immunogen: | Recombinant protein of human UBE2C |
| Post Translational Modifications | Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed. |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 C(E3-Independent E2 Ubiquitin-Conjugating Enzyme CE2 Ubiquitin-Conjugating Enzyme CUbch10Ubiquitin Carrier Protein CUbiquitin-Protein Ligase C |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-8866652Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 C antibodyAnti-(E3-Independent E2 Ubiquitin-Conjugating Enzyme C antibodyAnti-E2 Ubiquitin-Conjugating Enzyme C antibodyAnti-Ubch10 antibodyAnti-Ubiquitin Carrier Protein C antibodyAnti-Ubiquitin-Protein Ligase C antibodyAnti-UBE2C antibodyAnti-UBCH10 antibody |
Information sourced from Uniprot.org