Anti-UBE3C antibody (Internal) (STJ96167)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the Internal region of human UBE3C.
General Information
| Short Description | Rabbit polyclonal anti-Ubiquitin-protein ligase E3C (Internal) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:10000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE3C |
| Gene ID | 9690 |
| Uniprot ID | UBE3C_HUMAN |
| Immunogen | Synthesized peptide derived from the Internal region of human UBE3C. |
| Immunogen Region | Internal |
| Specificity | UBE3C Polyclonal Antibody detects endogenous levels of UBE3C protein. |
Additional Info
| Post Translational Modifications | Autoubiquitinated.promoting its own degradation. |
| Function | E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains. Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome. Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins. Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains.with some preference for 'Lys-48' linkages. Acts as a negative regulator of type I interferon by mediating 'Lys-48'-linked ubiquitination of IRF3 and IRF7, leading to their degradation by the proteasome. Catalyzes ubiquitination and degradation of CAND2. |
| Protein Name | Ubiquitin-Protein Ligase E3cHect-Type Ubiquitin Transferase E3cHomologous To E6ap Carboxyl Terminus Homologous Protein 2Hecth2Rta-Associated Ubiquitin LigaseRaul |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Ubiquitin-Protein Ligase E3c antibodyAnti-Hect-Type Ubiquitin Transferase E3c antibodyAnti-Homologous To E6ap Carboxyl Terminus Homologous Protein 2 antibodyAnti-Hecth2 antibodyAnti-Rta-Associated Ubiquitin Ligase antibodyAnti-Raul antibodyAnti-UBE3C antibodyAnti-KIAA0010 antibodyAnti-KIAA10 antibody |
Information sourced from Uniprot.org