Anti-UBE2I antibody (N-Term) (STJ70294)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-UBE2I (N-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 17kDa band observed in Human Kidney lysates and in Rat Ovary, Heart and Testis lysates (calculated MW of 18.0kDa according to Human NP_003336.1). Recommended concentration: 0.1- |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE2I |
| Gene ID | 7329 |
| Uniprot ID | UBC9_HUMAN |
| Accession Number | NP_003336.1 |
| Immunogen Region | N-Term |
| Immunogen Sequence | SGIALSRLAQERK |
| Specificity | Reported variants represent identical protein: NP_919237.1, NP_003336.1, NP_919236.1, NP_919235.1 |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-71 significantly enhances SUMOylation activity. |
| Function | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity. |
| Protein Name | Sumo-Conjugating Enzyme Ubc9Ring-Type E3 Sumo Transferase Ubc9Sumo-Protein LigaseUbiquitin Carrier Protein 9Ubiquitin Carrier Protein IUbiquitin-Conjugating Enzyme E2 IUbiquitin-Protein Ligase IP18 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-196791Reactome: R-HSA-3065678Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-4655427Reactome: R-HSA-4755510Reactome: R-HSA-5693565Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-8866904Reactome: R-HSA-9615933Reactome: R-HSA-9683610Reactome: R-HSA-9694631Reactome: R-HSA-9735871Reactome: R-HSA-9793242Reactome: R-HSA-9833482Reactome: R-HSA-9843940 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly NuclearIn SpermatocytesLocalizes In Synaptonemal ComplexesRecruited By Bcl11a Into The Nuclear Body |
| Alternative Antibody Names | Anti-Sumo-Conjugating Enzyme Ubc9 antibodyAnti-Ring-Type E3 Sumo Transferase Ubc9 antibodyAnti-Sumo-Protein Ligase antibodyAnti-Ubiquitin Carrier Protein 9 antibodyAnti-Ubiquitin Carrier Protein I antibodyAnti-Ubiquitin-Conjugating Enzyme E2 I antibodyAnti-Ubiquitin-Protein Ligase I antibodyAnti-P18 antibodyAnti-UBE2I antibodyAnti-UBC9 antibodyAnti-UBCE9 antibody |
Information sourced from Uniprot.org