• Immunofluorescence analysis of L929 cells using Thioredoxin reductase 2 (TXNRD2 ) antibody (STJ27443) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using Thioredoxin reductase 2 (TXNRD2 ) antibody (STJ27443) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Thioredoxin reductase 2 (Thioredoxin reductase 2 (TXNRD2 ) ) antibody (STJ27443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using Thioredoxin reductase 2 (Thioredoxin reductase 2 (TXNRD2 ) ) antibody (STJ27443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Thioredoxin reductase 2 (Thioredoxin reductase 2 (TXNRD2 ) ) antibody (STJ27443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TXNRD2 antibody (100-310) (STJ27443)

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STJ27443

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TXNRD2 (100-310) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TXNRD2
Gene ID: 10587
Uniprot ID: TRXR2_HUMAN
Immunogen Region: 100-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-310 of human Thioredoxin reductase 2 (Thioredoxin reductase 2 (TXNRD2 ) ) (NP_006431.2).
Immunogen Sequence: AALLGGLIQDAPNYGWEVAQ PVPHDWRKMAEAVQNHVKSL NWGHRVQLQDRKVKYFNIKA SFVDEHTVCGVAKGGKEILL SADHIIIATGGRPRYPTHIE GALEYGITSDDIFWLKESPG KTLVVGASYVALECAGFLTG IGLDTTIMMRSIPLRGFDQQ MSSMVIEHMASHGTRFLRGC APSRVRRLPDGQLQVTWEDS TTGKEDTGTFD
Tissue Specificity Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. high levels in kidney, whereas levels are low. High expression is observed in the adrenal cortex.
Function Involved in the control of reactive oxygen species levels and the regulation of mitochondrial redox homeostasis. Maintains thioredoxin in a reduced state. May play a role in redox-regulated cell signaling.
Protein Name Thioredoxin Reductase 2 - Mitochondrial
Selenoprotein Z
Selz
Tr-Beta
Thioredoxin Reductase Tr3
Database Links Reactome: R-HSA-3299685
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Thioredoxin Reductase 2 - Mitochondrial antibody
Anti-Selenoprotein Z antibody
Anti-Selz antibody
Anti-Tr-Beta antibody
Anti-Thioredoxin Reductase Tr3 antibody
Anti-TXNRD2 antibody
Anti-KIAA1652 antibody
Anti-TRXR2 antibody

Information sourced from Uniprot.org

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