• Immunofluorescence analysis of U-2 OS cells using TXNDC12 Rabbit polyclonal antibody (STJ116614) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using TXNDC12 Rabbit polyclonal antibody (STJ116614) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TXNDC12 in paraffin-embedded Rat brain using TXNDC12 Rabbit polyclonal antibody (STJ116614) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TXNDC12 in paraffin-embedded Human liver cancer using TXNDC12 Rabbit polyclonal antibody (STJ116614) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TXNDC12 Rabbit polyclonal antibody (STJ116614) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TXNDC12 antibody (93-172) (STJ116614)

SKU:
STJ116614

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TXNDC12
Gene ID: 51060
Uniprot ID: TXD12_HUMAN
Immunogen Region: 93-172
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 93-172 of human TXNDC12 (NP_056997.1).
Immunogen Sequence: NLEDEEEPKDEDFSPDGGYI PRILFLDPSGKVHPEIINEN GNPSYKYFYVSAEQVVQGMK EAQERLTGDAFRKKHLEDEL
Tissue Specificity Widely expressed.
Function Protein-disulfide reductase of the endoplasmic reticulum that promotes disulfide bond formation in client proteins through its thiol-disulfide oxidase activity.
Protein Name Thioredoxin Domain-Containing Protein 12
Endoplasmic Reticulum Resident Protein 18
Er Protein 18
Erp18
Endoplasmic Reticulum Resident Protein 19
Er Protein 19
Erp19
Thioredoxin-Like Protein P19
Htlp19
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Thioredoxin Domain-Containing Protein 12 antibody
Anti-Endoplasmic Reticulum Resident Protein 18 antibody
Anti-Er Protein 18 antibody
Anti-Erp18 antibody
Anti-Endoplasmic Reticulum Resident Protein 19 antibody
Anti-Er Protein 19 antibody
Anti-Erp19 antibody
Anti-Thioredoxin-Like Protein P19 antibody
Anti-Htlp19 antibody
Anti-TXNDC12 antibody
Anti-TLP19 antibody
Anti-UNQ713 antibody
Anti-PRO1376 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance