• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g TWF2 antibody (STJ11104301). Western blot was performed from the immunoprecipitate using TWF2 antibody (STJ11104301) at a dilution of 1:1000.
  • Western blot analysis of various lysates using TWF2 Rabbit polyclonal antibody (STJ11104301) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.

Anti-TWF2 antibody (1-369) (STJ11104301)

SKU:
STJ11104301

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Twinfilin-2 (1-369) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TWF2
Gene ID: 11344
Uniprot ID: TWF2_HUMAN
Immunogen Region: 1-369
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1).
Immunogen Sequence: MAHQTGIHATEELKEFFAKA RAGSVRLIKVVIEDEQLVLG ASQEPVGRWDQDYDRAVLPL LDAQQPCYLLYRLDSQNAQG FEWLFLAWSPDNSPVRLKML YAATRATVKKEFGGGHIKDE LFGTVKDDLSFAGYQKHLSS CAAPAPLTSAERELQQIRIN EVKTEISVESKHQTLQGLAF PLQPEAQRALQQLKQKMVNY IQMKLDLERETIELVHTEPT DVAQLPSRVPRDAARYHFF
Tissue Specificity Ubiquitously expressed (at protein level).
Post Translational Modifications In vitro, phosphorylated by PRKCZ, CK2 and SRC.
Function Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia.
Protein Name Twinfilin-2
A6-Related Protein
Ha6rp
Protein Tyrosine Kinase 9-Like
Twinfilin-1-Like Protein
Database Links Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cytoskeleton
Perinuclear Region
Cell Projection
Stereocilium
Perinuclear And G-Actin-Rich Cortical Actin Structure Sublocalization
Alternative Antibody Names Anti-Twinfilin-2 antibody
Anti-A6-Related Protein antibody
Anti-Ha6rp antibody
Anti-Protein Tyrosine Kinase 9-Like antibody
Anti-Twinfilin-1-Like Protein antibody
Anti-TWF2 antibody
Anti-PTK9L antibody
Anti-MSTP011 antibody

Information sourced from Uniprot.org

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