• Western blot analysis of extracts of HeLa cells, using TTK antibody (STJ25985) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH/3T3 cells using TTK rabbit polyclonal antibody (STJ25985) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using TTK rabbit polyclonal antibody (STJ25985) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-TTK antibody (536-789) (STJ25985)

SKU:
STJ25985

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:5000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TTK
Gene ID: 7272
Uniprot ID: TTK_HUMAN
Immunogen Region: 536-789
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 536-789 of human TTK (NP_003309.2).
Immunogen Sequence: SSKVFQVLNEKKQIYAIKYV NLEEADNQTLDSYRNEIAYL NKLQQHSDKIIRLYDYEITD QYIYMVMECGNIDLNSWLKK KKSIDPWERKSYWKNMLEAV HTIHQHGIVHSDLKPANFLI VDGMLKLIDFGIANQMQPDT TSVVKDSQVGTVNYMPPEAI KDMSSSRENGKSKSKISPKS DVWSLGCILYYMTYGKTPFQ QIINQISKLHAIIDPNHEIE FPDIPEKDLQDVLKCCLKR
Tissue Specificity Present in rapidly proliferating cell lines.
Function Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments. Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint. Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore. Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments. Phosphorylates KNL1, KNTC1 and autophosphorylates.
Protein Name Dual Specificity Protein Kinase Ttk
Phosphotyrosine Picked Threonine-Protein Kinase
Pyt
Alternative Antibody Names Anti-Dual Specificity Protein Kinase Ttk antibody
Anti-Phosphotyrosine Picked Threonine-Protein Kinase antibody
Anti-Pyt antibody
Anti-TTK antibody
Anti-MPS1 antibody
Anti-MPS1L1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance