• Western blot analysis of extracts of HeLa cells, using TTK antibody (STJ25985) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH/3T3 cells using TTK rabbit polyclonal antibody (STJ25985) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using TTK rabbit polyclonal antibody (STJ25985) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-TTK antibody (536-789) (STJ25985)

SKU:
STJ25985

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TTK (536-789) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TTK
Gene ID: 7272
Uniprot ID: TTK_HUMAN
Immunogen Region: 536-789
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 536-789 of human TTK (NP_003309.2).
Immunogen Sequence: SSKVFQVLNEKKQIYAIKYV NLEEADNQTLDSYRNEIAYL NKLQQHSDKIIRLYDYEITD QYIYMVMECGNIDLNSWLKK KKSIDPWERKSYWKNMLEAV HTIHQHGIVHSDLKPANFLI VDGMLKLIDFGIANQMQPDT TSVVKDSQVGTVNYMPPEAI KDMSSSRENGKSKSKISPKS DVWSLGCILYYMTYGKTPFQ QIINQISKLHAIIDPNHEIE FPDIPEKDLQDVLKCCLKR
Tissue Specificity Present in rapidly proliferating cell lines.
Function Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Phosphorylates MAD1L1 to promote mitotic checkpoint signaling. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Protein Name Dual Specificity Protein Kinase Ttk
Phosphotyrosine Picked Threonine-Protein Kinase
Pyt
Alternative Antibody Names Anti-Dual Specificity Protein Kinase Ttk antibody
Anti-Phosphotyrosine Picked Threonine-Protein Kinase antibody
Anti-Pyt antibody
Anti-TTK antibody
Anti-MPS1 antibody
Anti-MPS1L1 antibody

Information sourced from Uniprot.org

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