| Short Description : | Rabbit polyclonal TTK (536-789) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TTK |
| Gene ID: | 7272 |
| Uniprot ID: | TTK_HUMAN |
| Immunogen Region: | 536-789 |
| Immunogen Sequence: | SSKVFQVLNEKKQIYAIKYV NLEEADNQTLDSYRNEIAYL NKLQQHSDKIIRLYDYEITD QYIYMVMECGNIDLNSWLKK KKSIDPWERKSYWKNMLEAV HTIHQHGIVHSDLKPANFLI VDGMLKLIDFGIANQMQPDT TSVVKDSQVGTVNYMPPEAI KDMSSSRENGKSKSKISPKS DVWSLGCILYYMTYGKTPFQ QIINQISKLHAIIDPNHEIE FPDIPEKDLQDVLKCCLKR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 536-789 of human TTK (NP_003309.2). |
| Tissue Specificity | Present in rapidly proliferating cell lines. |
| Function | Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments. Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint. Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore. Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments. Phosphorylates KNL1, KNTC1 and autophosphorylates. Phosphorylates MCRS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and promotes chromosome alignment. |
| Protein Name | Dual Specificity Protein Kinase TtkPhosphotyrosine Picked Threonine-Protein KinasePyt |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Dual Specificity Protein Kinase Ttk antibodyAnti-Phosphotyrosine Picked Threonine-Protein Kinase antibodyAnti-Pyt antibodyAnti-TTK antibodyAnti-MPS1 antibodyAnti-MPS1L1 antibody |
Information sourced from Uniprot.org
