• Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells, using 3 Mu g TST antibody (STJ112560). Western blot was performed from the immunoprecipitate using TST antibody (STJ112560) at a dilution of 1:1000.
  • Immunohistochemistry analysis of TST in paraffin-embedded human thyroid cancer using TST Rabbit polyclonal antibody (STJ112560) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TST in paraffin-embedded human liver using TST Rabbit polyclonal antibody (STJ112560) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TST Rabbit polyclonal antibody (STJ112560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TST antibody (1-297) (STJ112560)

SKU:
STJ112560

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
IHC-P:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TST
Gene ID: 7263
Uniprot ID: THTR_HUMAN
Immunogen Region: 1-297
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).
Immunogen Sequence: MVHQVLYRALVSTKWLAESI RTGKLGPGLRVLDASWYSPG TREARKEYLERHVPGASFFD IEECRDTASPYEMMLPSEAG FAEYVGRLGISNHTHVVVYD GEHLGSFYAPRVWWMFRVFG HRTVSVLNGGFRNWLKEGHP VTSEPSRPEPAVFKATLDRS LLKTYEQVLENLESKRFQLV DSRSQGRFLGTEPEPDAVGL DSGHIRGAVNMPFMDFLTED GFEKGPEELRALFQTKKVD
Function Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
Protein Name Thiosulfate Sulfurtransferase
Rhodanese
Database Links Reactome: R-HSA-1614517
Reactome: R-HSA-1614558
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Thiosulfate Sulfurtransferase antibody
Anti-Rhodanese antibody
Anti-TST antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance