| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TSEN2 |
| Gene ID: | 80746 |
| Uniprot ID: | SEN2_HUMAN |
| Immunogen Region: | 1-220 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TSEN2 (NP_079541.1). |
| Immunogen Sequence: | MAEAVFHAPKRKRRVYETYE SPLPIPFGQDHGPLKEFKIF RAEMINNNVIVRNAEDIEQL YGKGYFGKGILSRSRPSFTI SDPKLVAKWKDMKTNMPIIT SKRYQHSVEWAAELMRRQGQ DESTVRRILKDYTKPLEHPP VKRNEEAQVHDKLNSGMVSN MEGTAGGERPSVVNGDSGKS GGVGDPREPLGCLQEGSGCH PTTESFEKSVREDASPLPHV |
| Tissue Specificity | Isoform 1 and isoform 2 are widely expressed at very low level. |
| Function | Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. |
| Protein Name | Trna-Splicing Endonuclease Subunit Sen2 Trna-Intron Endonuclease Sen2 Hssen2 |
| Database Links | Reactome: R-HSA-6784531 |
| Cellular Localisation | Nucleus Nucleolus May Be Transiently Localized In The Nucleolus |
| Alternative Antibody Names | Anti-Trna-Splicing Endonuclease Subunit Sen2 antibody Anti-Trna-Intron Endonuclease Sen2 antibody Anti-Hssen2 antibody Anti-TSEN2 antibody Anti-SEN2 antibody |
Information sourced from Uniprot.org