| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TSC1 (600-700) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:100IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TSC1 |
| Gene ID: |
7248 |
| Uniprot ID: |
TSC1_HUMAN |
| Immunogen Region: |
600-700 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 600-700 of human TSC1 (NP_000359.1). |
| Immunogen Sequence: |
QPPPYDHLFEVALPKTAHHF VIRKTEELLKKAKGNTEEDG VPSTSPMEVLDRLIQQGADA HSKELNKLPLPSKSVDWTHF GGSPPSDEIRTLRDQLLLLH N |
| Tissue Specificity | Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells. |
| Post Translational Modifications | Phosphorylation at Ser-505 does not affect interaction with TSC2. |
| Function | Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. The TSC-TBC complex acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Within the TSC-TBC complex, TSC1 stabilizes TSC2 and prevents TSC2 self-aggregation. Acts as a tumor suppressor. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1. Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1. |
| Protein Name | HamartinTuberous Sclerosis 1 Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165181Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-8854214 |
| Cellular Localisation | Lysosome MembranePeripheral Membrane ProteinCytoplasmCytosolRecruited To Lysosomal Membranes In A Rheb-Dependent Process In Absence Of NutrientsIn Response To NutrientsThe Complex Dissociates From Lysosomal Membranes And Relocalizes To The Cytosol |
| Alternative Antibody Names | Anti-Hamartin antibodyAnti-Tuberous Sclerosis 1 Protein antibodyAnti-TSC1 antibodyAnti-KIAA0243 antibodyAnti-TSC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
