Anti-Tryptophan tRNA Ligase antibody [R09-1D2] (STJA0033792)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Tryptophanyl tRNA synthetase
STJA0033792
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General Information

Short DescriptionRabbit monoclonal anti-Tryptophan tRNA Ligase for use in WB, IHC-F, IHC-P, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-F/IHC-P/ICC/IF/IP
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR09-1D2
IsotypeIgG
ConjugationUnconjugated
Concentration0.3 mg/mL
PurificationAffinity Purified
Dilution RangeWB 1:500-1:1000
IHC 1:50-1:100
IF 1:50-1:200
IP 1:20
Formulation50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolWARS1
Gene ID7453
Uniprot IDSYWC_HUMAN
ImmunogenA synthetic peptide of human Tryptophanyl tRNA synthetase

Additional Info

Post Translational Modifications Proteolytic cleavage generates 2 forms.T1-TrpRS and T2-TrpRS.
Function Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp). Isoform 1: Has no angiostatic activity. T2-TrpRS: Possesses an angiostatic activity but has no aminoacylation activity. Inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Isoform 2: Has an angiostatic activity.
Protein Name Tryptophan--Trna Ligase - Cytoplasmic
Interferon-Induced Protein 53
Ifp53
Tryptophanyl-Trna Synthetase
Trprs
Hwrs Cleaved Into - T1-Trprs - T2-Trprs
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Tryptophan--Trna Ligase - Cytoplasmic antibody
Anti-Interferon-Induced Protein 53 antibody
Anti-Ifp53 antibody
Anti-Tryptophanyl-Trna Synthetase antibody
Anti-Trprs antibody
Anti-Hwrs Cleaved Into - T1-Trprs - T2-Trprs antibody
Anti-WARS1 antibody
Anti-IFI53 antibody
Anti-WARS antibody
Anti-WRS antibody

Information sourced from Uniprot.org

Citations

Product Review