| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRPM7 (Extracellular region) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Antigen Affinity Purified |
| Dilution Range: |
WB 1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRPM7 |
| Gene ID: |
54822 |
| Uniprot ID: |
TRPM7_HUMAN |
| Immunogen Region: |
Extracellular region |
| Specificity: |
This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MCF7, MDA-MB-231, mouse brain, and rat PC12 cells. |
| Immunogen: |
TRPM7 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members. |
| Function | Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. The channel is highly permeable to divalent cations, specifically calcium (Ca2+), magnesium (Mg2+) and zinc (Zn2+) and mediates their influx. Controls a wide range of biological processes such as Ca2(+), Mg(2+) and Zn(2+) homeostasis, vesicular Zn(2+) release channel and intracellular Ca(2+) signaling, embryonic development, immune responses, cell motility, proliferation and differentiation. The C-terminal alpha-kinase domain autophosphorylates cytoplasmic residues of TRPM7. In vivo, TRPM7 phosphorylates SMAD2, suggesting that TRPM7 kinase may play a role in activating SMAD signaling pathways. In vitro, TRPM7 kinase phosphorylates ANXA1 (annexin A1), myosin II isoforms and a variety of proteins with diverse cellular functions. TRPM7 channel, cleaved form: The cleaved channel exhibits substantially higher current and potentiates Fas receptor signaling. TRPM7 kinase, cleaved form: The C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. In immune cells, the TRPM7 kinase domain is clipped from the channel domain by caspases in response to Fas-receptor stimulation. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins in a Zn(2+)-dependent manner to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and embryonic development. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 7Channel-Kinase 1Long Transient Receptor Potential Channel 7Ltrpc-7Ltrpc7 Cleaved Into - Trpm7 Kinase - Cleaved FormM7ck - Trpm7 Channel - Cleaved Form |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLocalized Largely In Intracellular Zn(2+)-Storage VesiclesTrpm7 KinaseCleaved Form: Nucleus |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 7 antibodyAnti-Channel-Kinase 1 antibodyAnti-Long Transient Receptor Potential Channel 7 antibodyAnti-Ltrpc-7 antibodyAnti-Ltrpc7 Cleaved Into - Trpm7 Kinase - Cleaved Form antibodyAnti-M7ck - Trpm7 Channel - Cleaved Form antibodyAnti-TRPM7 antibodyAnti-CHAK1 antibodyAnti-LTRPC7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
