| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-TRPM2 (1204-1503) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRPM2 |
| Gene ID: |
7226 |
| Uniprot ID: |
TRPM2_HUMAN |
| Immunogen Region: |
1204-1503 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1204-1503 of human TRPM2 (NP_003298.1). |
| Immunogen Sequence: |
SSEADVPTLASQKAAEEPDA EPGGRKKTEEPGDSYHVNAR HLLYPNCPVTRFPVPNEKVP WETEFLIYDPPFYTAERKDA AAMDPMGDTLEPLSTIQYNV VDGLRDRRSFHGPYTVQAGL PLNPMGRTGLRGRGSLSCFG PNHTLYPMVTRWRRNEDGAI CRKSIKKMLEVLVVKLPLSE HWALPGGSREPGEMLPRKLK RILRQEHWPSFENLLKCGME VYKGYMDDPRNTDNAWIET |
| Tissue Specificity | Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes. |
| Function | Isoform 1: Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels. Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change.the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity. Contributes to Ca(2+) release from intracellular stores in response to ADP-ribose. Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels. Activated by moderate heat (35 to 40 degrees Celsius). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species. The precise physiological activators are under debate.the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels. Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels. Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca(2+) and Zn(2+) levels. Confers susceptibility to cell death following oxidative stress. Isoform 2: Lacks cation channel activity. Does not mediate cation transport in response to oxidative stress or ADP-ribose. Isoform 3: Lacks cation channel activity and negatively regulates the channel activity of isoform 1. Negatively regulates susceptibility to cell death in reposponse to oxidative stress. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 2Estrogen-Responsive Element-Associated Gene 1 ProteinLong Transient Receptor Potential Channel 2Ltrpc-2Ltrpc2Transient Receptor Potential Channel 7Trpc7Transient Receptor Potential Melastatin 2 |
| Database Links | Reactome: R-HSA-3295583Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinPerikaryonCell ProjectionCytoplasmic VesicleLysosomeDetected At The Cell Membrane And In Intracellular Vesicles In Cortical NeuronsDetected On Neuronal Cell Bodies And NeuritesDetected On The Cell Membrane In Polymorphonuclear NeutrophilsDetected On Cytoplasmic Vesicles And Lysosomes In Immature Bone Marrow Dendritic CellsIsoform 1: Cell MembraneIsoform 2: Cell MembraneIsoform 3: Cell Membrane |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 2 antibodyAnti-Estrogen-Responsive Element-Associated Gene 1 Protein antibodyAnti-Long Transient Receptor Potential Channel 2 antibodyAnti-Ltrpc-2 antibodyAnti-Ltrpc2 antibodyAnti-Transient Receptor Potential Channel 7 antibodyAnti-Trpc7 antibodyAnti-Transient Receptor Potential Melastatin 2 antibodyAnti-TRPM2 antibodyAnti-EREG1 antibodyAnti-KNP3 antibodyAnti-LTRPC2 antibodyAnti-TRPC7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
