| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-TRPA1 (4th Cytoplasmic Loop) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
TRPA1 |
| Gene ID: |
8989 |
| Uniprot ID: |
TRPA1_HUMAN |
| Immunogen Region: |
4th Cytoplasmic Loop |
| Specificity: |
Specific for TRPA1. |
| Immunogen: |
A synthetic from the 4th cytoplasmic loop of human transient receptor potential cation channel subfamily A member 1 (TRPA1, ANKTM1) conjugated to an immunogenic carrier protein was used as the antigen. |
| Function | Ligand-activated Ca(2+)-permeable, nonselective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Has a relatively high Ca(2+) selectivity, with a preference for divalent over monovalent cations (Ca(2+) > Ba(2+) > Mg(2+) > NH4(+) > Li(+) > K(+)), the influx of cation into the cytoplasm leads to membrane depolarization. Has a central role in the pain response to endogenous inflammatory mediators, such as bradykinin and to a diverse array of irritants. Activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds, such as allylthiocyanate (AITC) from mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an environmental irritant. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner. Non-electrophile agonists bind at distinct sites in the transmembrane domain to promote channel activation. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily A Member 1Ankyrin-Like With Transmembrane Domains Protein 1Transformation-Sensitive Protein P120P120Wasabi Receptor |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily A Member 1 antibodyAnti-Ankyrin-Like With Transmembrane Domains Protein 1 antibodyAnti-Transformation-Sensitive Protein P120 antibodyAnti-P120 antibodyAnti-Wasabi Receptor antibodyAnti-TRPA1 antibodyAnti-ANKTM1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
